igScript™ One Step RT-qPCR kit combines two powerful mixtures: i). igScript™ Reverse Transcriptase and ii) ig SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.
igScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42-55ºC.
Intact Genomics SYBR® Green qPCR 2x master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.
Applications
- Gene expression data validation.
- Multiplexing
- Mutation detection
- Pathogen and viral detection
- Genetically modified organisms (GMO) characterization and Genetic profiling
Benefits
- Enhanced efficiency, specificity, and sensitivity
- Compatible with all real-time PCR instruments
- Superior gene expression results under various cycling conditions
- Robust and active for cDNA synthesis at temperatures up to 55°C.
igScript™ One Step RT-qPCR Kit Includes
- ig Script™ Reverse Transcriptase
- ig SYBR™ Green qPCR 2x Master Mix
Storage Temperature–20 °C4214 4218
