Intact Genomics (ig^®) EHA105 (pSoup) ElectroCompetent Agrobacterium cells are optimized for the highest transformation efficiencies ideal for applications with cDNA or gDNA library construction. EHA105 (pSoup) contains a rifampicin resistance gene (rif).  EHA105 (pSoup) strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which contains the vir gene. The pSoup plasmid aids in replication of pGreen, 62SK, and pGs2 series plasmids. This system is used for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.

SpecificationsCompetent cell type: ElectrocompetentSpecies: A. tumefaciensStrain: EHA105 (pSoup)Format: TubesTransformation efficiency:  ≥ 1 x 10⁷  cfu/µg pCAMBIA1391z DNABlue/white screening: NoShipping condition: Dry ice

Reagents Needed for One ReactionEHA105 ElectroComp Agrobacterium: 25 µlDNA (pCAMBIA1391z, 500 pg/µl):    1 µlRecovery medium:    1 ml

StorageEHA105 (pSoup) ElectroComp Agrobacterium: -80 ºCpCAMBIA1391z control DNA: -20 ºCRecovery medium:    4 ºC

Genotype

C58 (rif^R), Ti pEHA105 (pTiBo542DT-DNA), pSOUP (tet^R), Succinamopine

Quality ControlTransformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ≥1 x 10⁷ CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

Please note, all agrobacterial strains are not well studied for antibiotic resistance and there are many agrobacterial strains.  Therefore, it is the customer’s responsibility to make sure his/her vectors are compatible with the Agrobacterial strains if he/she uses an alternate antibiotic selection than kanamycin-selection.

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