Intact Genomics (ig®) C58C1 ElectroCompetent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. These competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin-resistant.
SpecificationsCompetent cell type: ElectrocompetentSpecies: A. tumefaciensStrain: C58C1Format: TubesTransformation efficiency: ≥ 1 x 107 cfu/µg pCAMBIA1391z DNABlue/white screening: NoShipping condition: Dry ice
Reagents Needed for One ReactionC58C1 ElectroCompetent Agrobacterium: 25 µlDNA (pCAMBIA1391z, 500 pg/µl): 1 µlRecovery medium: 1 ml
StorageC58C1 ElectroCompetent Agrobacterium: -80 ºCpCAMBIA1391z control DNA: -20 ºCRecovery medium: 4 ºC
Quality ControlTransformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ≥1 x 107 CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
Please note, all agrobacterial strains are not well-studied for antibiotic resistance and there are many agrobacterial strains. Therefore, it is the customer’s responsibility to make sure his/her vectors are compatible with the Agrobacterial strains if he/she uses an alternate antibiotic selection than kanamycin-selection.
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Technical Support
Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.
