Intact Genomics (ig^®) DirectPlate-XL™ DH10B Competent cells offer simple, fast, and robust results for your large DNA transformation needs – directly clone large DNA up to >250kb! DirectPlate-XL™ DH10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their large DNA transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate-XL™ DH10B chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.  Using proprietary technology, these cells can be used for the transformation of extremely large DNA (i.e. >10kb up to >250kb).

Please find a video below displaying the convenience and capability of this product.

Specifications

Competent cell type:  Chemically competentDerivative of:  DH10BSpecies:  E. coliFormat: TubesTransformation efficiency: ≥1 x 10⁸ – 10⁹ cfu/µg pUC19 DNABlue/white screening: YesShipping condition: Dry ice

Reagents Needed for One Reaction

DirectPlate-XL™ DH10B competent cell: 50 µlDNA (or pUC19 Control, 10 pg/µl): 1 µl

Storage

DirectPlate-XL™ DH10B competent cells: -80 ºCpUC19 control DNA:  -20 ºC

Genotype

F – mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara, leu)7697 galU galK rpsL (StrR) nupG λ-

Quality Control

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be greater than 1×10⁸ – 10⁹ CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

Follow these guidelines when using DirectPlate-XL™ DH10B chemically competent E. coli.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by gently pipetting up and down a few times. 1013-13 1013-36

Comparison Testing

Intact Genomics has performed extensive comparison testing to prove our DirectPlate-XL™ DH10B chemically competent cells have the highest transformation efficiency on the market.

Additional experimental details are available upon request.1013-13 1013-36