Intact Genomics (ig^®) GV3101 (pSoup) ElectroCompetent Agrobacterium cells are optimized for the highest transformation efficiencies, ideal for applications with cDNA or gDNA library construction.  The GV3101 (pSoup) strain has resistance to rifampicin, gentamicin, and tetracyline (see genotype). The GV3101 Ti plasmid has the T-DNA region deleted and transformation with a binary vector containing the missing T-region restores the T-DNA binary system function. The pSoup plasmid aids in replication of pGreen, 62SK, and pGs2 series plasmids. This system is used for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.

SpecificationsCompetent cell type: ElectrocompetentSpecies: A. tumefaciensStrain: GV3101 (pSoup)Format: TubesTransformation efficiency:  ≥ 1 x 10⁷  cfu/µg pCAMBIA1391z  DNABlue/white screening: NoShipping condition: Dry ice

Reagents Needed for One ReactionGV3101 (pSoup) ElectroComp Agrobacterium: 25 µlDNA (pCAMBIA1391z, 500 pg/µl):    1 µlRecovery medium:    1 ml

StorageGV3101 (pSoup) ElectroComp Agrobacterium: -80 ºCpCAMBIA1391z control DNA: -20 ºCRecovery medium:    4 ºC

GenotypeC58 (rif^R), Ti pMP90 (pTiC58DT-DNA) ( gent^R), pSOUP (tet^R), Nopaline

Quality ControlTransformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ≥1 x 10⁷ CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

Please note, all agrobacterial strains are not well studied for antibiotic resistance and there are many agrobacterial strains.  Therefore, it is the customer’s responsibility to make sure his/her vectors are compatible with the Agrobacterial strains if he/she uses an alternate antibiotic selection than kanamycin-selection.1282PS-12 1282PS-36