Intact Genomics JM109-DE3 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. JM109-DE3 is useful for the high-level expression of genes cloned into vectors for the expression of sequence downstream from the T7 promoter.
SpecificationsCompetent cell type: Chemically competentDerivative of: JM109Species: E. coliFormat: TubesTransformation efficiency: ≥1.0 x 108 cfu/µg pUC19 DNABlue/white screening: YesShipping condition: Dry ice
Product Includes & Storage1) ig™ JM109(DE3) competent cells: -80 ºC2) pUC19 control DNA: -20 ºC3) Recovery medium: 4 ºC
Genomic Featuresig™ JM109-DE3 chemically competent cells have the following features:
- recA– and endA– to minimize recombination and improve the quality of plasmid DNA.
- Contains F’ episome for blue/white screening.
GenotypeendA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, λ-, Δ(lac–proAB), [F’ traD36, proAB, laqIqZΔM15], IDE3
Quality ControlTransformation efficiency is tested by using the pUC19 control DNA supplied with the kit and the high-efficiency transformation protocol listed below. Transformation efficiency should be ≥1 x 108 CFU/µg pUC19 DNA.
Technical Support
Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.
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