RNase inhibitors are commonly used to inhibit and control RNase contaminants in enzymatic manipulations of RNA. Intact Genomics RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. Moreover, it does not inhibit polymerase activity when used with Taq DNA Polymerase, Reverse Transcriptase. This Inhibitor from Murine is resistance to oxidation compared to the human/porcine inhibitors as it lacks the pair of cysteines found in the human/porcine versions. In addition, it is stable at less than 1 mM DTT concentration.
Protein PurityThe physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).
Product SourceE. coli strain expressing recombinant RNase Inhibitor gene from mouse.
Product Includes
- RNase Inhibitor, Murine
Applications
- cDNA synthesis
- RT-PCR/RT-qPCR
- In vitro transcription/translation
- RNA labeling
Unit DefinitionOne unit is defined as the amount of the Murine Inhibitor is required to inhibit the activity of 5 ng of RNase A by 50%.
Storage Temperature–20ºC
Heat Inactivation70ºC for 20 minutes
Storage Buffer50 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTA50% GlycerolpH 7.5 @ 25ºC
Storage Temperature–20ºC
Quality Control assaysSDS-PAGE purity, protein concentration, endo-deoxyribonuclease assay, performance evaluation with RT-qPCR
RNase Inhibitor (RI) Data ComparisonRT-qPCR was run using Intact Genomics (IG) 1-step 2x master mix (MM) and compared IG RI with a leading brand’s “N” RI.
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