Intact Genomics propriety ig-Fusion™ cloning technology is a simple, rapid, and highly efficient cloning kit that allows you to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high-fidelity Pfu DNA polymerase or other high-fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product needs to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 min) with more than 95% cloning efficiency.
Applications
- Clone any insert at any site within any vector
- Restriction enzyme and phosphatase-free system
- Joining multiple large fragments at once
- Precise insertion at a desired orientation
- Rapid and high efficiency with > 95% positive clones
Product Includes
- 5x ig-Fusion™ enzyme premix*
- 2x PCR premix
- High efficiency competent cell*5x ig-Fusion™ enzyme premix also sold separatelyClick here for 5x ig-Fusion™ enzyme premix
Storage Temperature
5x ig-Fusion™ enzyme premix: -20 ºC2x PCR premix: -20 ºCHigh-efficiency chemical competent cells: -80 ºC
Technical Support
Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.
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