Intact Genomics GV3101 ElectroCompetent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.
The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.
SpecificationsCompetent cell type: ElectrocompetentSpecies: A. tumefaciensStrain: GV3101Format: TubesTransformation efficiency: ≥ 1 x 107 cfu/µg pCAMBIA1391z DNABlue/white screening: NoShipping condition: Dry ice
Reagents Needed for One ReactionGV3101 ElectroComp Agrobacterium: 25 µlDNA (pCAMBIA1391z, 500 pg/µl): 1 µlRecovery medium: 1 ml
StorageGV3101 ElectroComp Agrobacterium: -80 ºCpCAMBIA1391z control DNA: -20 ºCRecovery medium: 4 ºC
Quality ControlTransformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ≥1 x 107 CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
Please note, all agrobacterial strains are not well studied for antibiotic resistance and there are many agrobacterial strains. Therefore, it is the customer’s responsibility to make sure his/her vectors are compatible with the Agrobacterial strains if he/she uses an alternate antibiotic selection than kanamycin-selection.1282-12 1282-20 1282-36
