Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig® 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.
Specifications
Competent cell type: Chemically competentDerivative of: 5-alphaSpecies: E. coliFormat: TubesTransformation efficiency: ≥1 x 109 cfu/µg pUC19 DNABlue/white screening: YesShipping condition: Dry ice
Reagents Needed for One Reaction
ig® 5-alpha chemically competent cells: 50 µlDNA (or pUC19 Control, 10 pg/µl): 1 µlRecovery medium: 1 ml
Storage
ig® 5-alpha competent cells: -80 ºCpUC19 control DNA: -20 ºCRecovery medium: 4 ºC
Genotype
Φ80 Δ(lacZ)M15 fhuA2 Δ(argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17
Quality Control
Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high efficiency transformation protocol. Transformation efficiency should be greater than 1×109 CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.
General Guidelines
Follow these guidelines when using ig™ 5-alpha chemically competent cells.
- Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
- Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.
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