Intact Genomics (ig^®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ 10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ 10B chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. Our DirectPlate™ 10B chemically competent cells have the highest transformation efficiency on the market.

Please find a video below displaying the convenience and capability of this product.

Specifications

Competent cell type:  Chemically competentDerivative of:  DH10BSpecies:  E. coliFormat:  TubesTransformation efficiency:  ≥1 x 10⁸ – 10⁹ cfu/µg pUC19 DNABlue/white screening:  YesShipping condition:  Dry ice

Reagents Needed for One Reaction

DirectPlate™ 10B competent cell:  50 µlDNA (or pUC19 Control, 10 pg/µl):  1 µl

Storage

DirectPlate™  10B competent cells:  -80 ºCpUC19 control DNA:  -20 ºC

Genotype

F – mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) nupG λ-

Quality Control

Transformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be greater than 1×10⁸ – 10⁹ CFU/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

General Guidelines

Follow these guidelines when using DirectPlate™  10B chemically competent E. coli.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by gently pipetting up and down a few times. 1013-13 1013-36

Comparison Testing

Intact Genomics has performed extensive comparison testing to prove our DirectPlate™  10B chemically competent cells have the highest transformation efficiency on the market.1015-12 1015-36