T4 gp32 Protein is a single-stranded DNA binding protein required for T4 DNA replication, recombination, and repair (1, 2). It improves the efficiency of reverse transcriptase (RT) during RT-PCR (3), enhances T4 DNA polymerase activity (4), as well as increases the yield of PCR products (5).
Note:Glycerol acts as a cryoprotectant and protein stabilizer when added to the storage buffer of proteins and enzymes. However, in certain circumstances, it is preferred to omit glycerol from the buffer. This includes instances where the presence of glycerol may interfere, such as lyophilization, high-throughput instruments with sensitive fluidics or primary cell cultures.For our glycerol free products, it is recommended to use immediately upon thaw as without glycerol there will be significant activity loss with freeze/thaw cycles.
Protein Purity
The physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).
Product Source
- coli BL21 (DE3) strain expressing T4 gp32 gene.
Product Includes
- Glycerol Free T4 gp32 protein
- 10X gp32 reaction buffer
1x GP 32 reaction buffer composition
20 mM Tris-acetate100 mM Potassium acetate10 mM Magnesium acetate1 mM DTTpH 7.8 @ 25ºC
Storage Buffer
50 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTApH 7.5 @ 25ºC
Storage Temperature
–20ºC
Heat Inactivation
65°C for 20 min
Quality Control assays
T4 gp32 protein is free from detectable nuclease activities.
References
- Chase, J. W. and Williams, K. R. (1986) Ann. Rev. Biochem. 55, 103-136
- Sinha, N. K. and Snustad, D. P. (1971) J. Mol. Biol. 62, 267-271.
- Baugh, L.R. et al. (2001). Nucl. Acids Res. 29, e29.
- Topal, M.D. and Sinha, N.K. (1983). J. Biol. Chem. 258, 12274-12279.
- Schwartz, K. et al. (1990). Nucl. Acids Res. 18, 1079.
3512 3515
