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当前位置: 首页 > 产品中心 > > intactgenomics/Glycerol Free T4 UvsY Protein |ig| Intact Genomics/1000µg (2μg/μl)/3577G
商品详细intactgenomics/Glycerol Free T4 UvsY Protein |ig| Intact Genomics/1000µg (2μg/μl)/3577G
intactgenomics/Glycerol Free T4 UvsY Protein |ig| Intact Genomics/1000µg (2μg/μl)/3577G
intactgenomics/Glycerol Free T4 UvsY Protein |ig| Intact Genomics/1000µg (2μg/μl)/3577G
商品编号: 3577G
品牌: ig
市场价: ¥34300.00
美元价: 17150.00
产地: 美国(厂家直采)
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产品分类: 其他
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联系Q Q: 3392242852
电话号码: 4000-520-616
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商品介绍

UvsY is the phage T4 recombination mediator protein, and structural and biophysical studies provide insights into its role in T4 homologous recombination. During T4 homologous recombination, the UvsX recombinase must compete with the prebound gp32 single-stranded binding protein for DNA-binding sites and T4 UvsY Protein stimulates this filament nucleation event (1). UvsY is a 15.8-kDa protein with properties that are consistent with its role as mediator; it stimulates the DNA-dependent ATPase activity of UvsX, lowers the critical concentration of UvsX that is required for activity, and promotes strand exchange (2, 3). UvsY efficiently promotes the UvsX-catalyzed strand invasion reaction by recruiting to ssDNA–gp32 complexes, promotes the release of gp32, and favors the binding of UvsX.

Note:Glycerol acts as a cryoprotectant and protein stabilizer when added to the storage buffer of proteins and enzymes. However, in certain circumstances, it is preferred to omit glycerol from the buffer. This includes instances where the presence of glycerol may interfere, such as lyophilization, high-throughput instruments with sensitive fluidics or primary cell cultures.For our glycerol free products, it is recommended to use immediately upon thaw as without glycerol there will be significant activity loss with freeze/thaw cycles.

Protein PurityThe physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).T4 UvsY Protein Purity

Product SourceE. coli BL21 (DE3) strain expressing T4 UvsY gene with an Nterminal His tag.This GST tag does not have any effect on UvsY activity.

Product IncludesGlycerol Free T4 UvsY protein10X UvsY Reaction Buffer

1x UvsY reaction buffer composition20 mM Tris-acetate100 mM Potassium acetate10 mM Magnesium acetate1 mM DTTpH 7.8 @ 25ºC

Storage Buffer50 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTApH 7.5 @ 25ºC

Storage Temperature-20ºC

Quality Control assaysGlycerol Free T4 UvsY is free from detectable nuclease activities.

References1. Beernink HT, Morrical SW. RMPs: Recombination/replication mediator proteins. Trends Biochem Sci. 1999;24(10):385–389.2. Hashimoto K, Yonesaki T. The characterization of a complex of three bacteriophage T4 recombination proteins, uvsX protein, uvsY protein, and gene 32 protein, on single-stranded DNA. JBiol Chem. 1991;266(8):4883–4888.3. Beernink HT, Morrical SW. The uvsY recombination protein of bacteriophage T4 forms hexamers in the presence and absence of single-stranded DNA. Biochemistry. 1998;37(16):5673– 6813572G 3575G 3577G

品牌介绍
Intact Genomics(ig)是大DNA片段克隆和宏基因组技术的世界领导者。我们有专门的科学家和最先进的设备,位于西线工业园,靠近拜耳作物科学总部、唐纳德·丹佛斯植物科学中心和圣路易斯的华盛顿大学。我们提供高质量的生命科学产品,包括高质量的感受态细胞、强健的酶/蛋白质、大插入DNA克隆、操作和BAC文库相关服务,以帮助科学家探索微生物、植物和动物物种的基因组结构和功能。我们经验丰富的科学家团队提供卓越的个性化服务、快速的周转时间和业内最合理的价格。我们还在宏基因组研究中应用BAC相关技术,为农业研究、天然产物酶发现和环境保护开发新的解决方案。在完整基因组公司,我们的专家是高分子量(HMW)基因组DNA制备、大DNA片段操作、无偏细菌人工染色体(BAC)文库构建、蛋白质表达和纯化领域的世界级领导者。我们持续关注基因组技术创新。